Comparison of UV C Light and Chemicals for Disinfection of Surfaces in Hospital Isolation Units

B. M. Andersen, MD, PhD; H. Banrud, Dr Scient; E. Boe, Bc Econ, MEng; O. Bjordal, MEng; F. Drangsholt, PhD





To determine the bactericidal effect on surfaces of ceiling‐ and wall‐mounted UV C (UVC) light (wavelength, 254 nm) in isolation units, compared with standard hospital environmental cleaning and chemical disinfection during final disinfection after patients are treated for infections.



Microbial samples were obtained from surfaces in isolation units (patient room, anteroom, and bathroom) before and after irradiation with UVC, chloramine disinfection, and standard hospital environmental cleaning. Samples were tested using standard contact plates.



Four identical, negative air‐pressure isolation units (patient room, anteroom, and bathroom) with a defined number of ceiling‐ and wall‐mounted UVC light units. The UVC distribution was monitored in one isolation unit after irradiation for approximately 40 minutes, corresponding to doses ranging from 160 J/m2 in a shadowed area to 19,230 J/m2 at the mostly highly exposed site (which is high enough to inactivate most bacterial organisms, including spores).



UVC disinfection significantly reduced the number of bacteria on surfaces directly or indirectly exposed to UVC to a very low number, as did 5% chloramine disinfection alone (for both). Completely shadowed areas in the isolation unit (eg, the bed rail, lockers, and mattresses) still required disinfection by chemicals.



Disinfection with UVC light may significantly reduce environmental bacterial contamination and thereby protect the next patient housed in an isolation room. UVC disinfection may not be used alone but is a good addition to chemical disinfection.


Infect Control Hosp Epidemiol 2006;27:729–734
© 2006 by The Society for Healthcare Epidemiology of America. All rights reserved.
DOI: 10.1086/503643






B. M. Andersen, MD, PhD;

H. Bånrud, DrScient;

E. Bøe, BcEcon, MEng;

O. Bjordal, MEng;

F. Drangsholt, PhD.

From the Department of Hospital infections and Department of Internal Services, Ullevål University Hospital, Oslo (B.M.A., E.B.); Klean ASA, Rud (H.B., O.B.); and the Faculty of Technology, Sør‐Trøndelag University College, Trondheim (F.D.), Norway.


Comparison of UV C Light and Chemicals for Disinfection of Surfaces in Hospital Isolation Units:  Created on May 24th, 2010.  Last Modified on May 24th, 2010


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