Resistance of Surface-dried Virus to Common Disinfection Procedures

Terpstra FG; van den Blink AE; Bos LM; Boots AG; Brinkhuis FH; Gijsen E; van Remmerden Y; Schuitemaker H; van 't Wout AB


It is believed that surface-dried viruses can remain infectious and may therefore pose a threat to public health. To help address this issue, we studied 0.1 N NaOH and 0.1% hypochlorite for their capacity to inactivate surface-dried lipid-enveloped (LE) [human immunodeficiency virus (HIV), bovine viral diarrhoea virus (BVDV) and pseudorabies virus (PRV)] and non-lipid-enveloped [NLE; canine parvovirus (CPV) and hepatitis A virus (HAV)] viruses in a background of either plasma or culture medium. In addition, 80% ethanol was tested on surface-dried LE viruses. Without treatment, surface-dried LE viruses remained infectious for at least one week and NLE viruses for more than one month. Irrespective of the disinfectant, inactivation decreased for viruses dried in plasma, which is more representative of viral contaminated blood than virus in culture medium. Inactivation by all disinfectants improved when preceded by rehydration, although the infectivity of CPV actually increased after rehydration and disinfection may thus be overestimated in the absence of rehydration. This is the first comprehensive study of five important (model) viruses in a surface-dried state showing persistence of infectivity, resistance to three commonly used disinfectants and restoration of susceptibility after rehydration. Our results may have implications for hygiene measurements in the prevention of virus transmission.


Resistance of surface-dried virus to common disinfection procedures.
Terpstra FG - J Hosp Infect - 01-AUG-2007; 66(4): 332-8




Source Title


The Journal of Hospital Infection


Author Affiliation


Sanquin Research, Landsteiner Laboratory, Center for Infection and Immunity Amsterdam, Academic Medical Center of the University of Amsterdam, Amsterdam, The Netherlands. [email protected]


Resistance of Surface-dried Virus to Common Disinfection Procedures:  Created on December 22nd, 2008.  Last Modified on July 28th, 2010


You must be logged in to comment:

Forgot your password?   Don't have an account?

Subscribe to CIRI's Newsletter


Stay in touch with cleaning-science news from CIRI. As a bonus, we’ll also give you a copy of the Frank Porter Graham Building Study - the foundation for most current high performance cleaning programs



First Name:
Last Name:
Email Address:




CIRI respects your privacy: (Privacy Policy) Account Registration

Fill in the form below for basic site access (commenting, etc).
Looking for more? Become a CIRI Member...