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Cholera Hides in Biofilm
"The germ that causes cholera (Vibrio cholerae) forms a biofilm that enables it to survive in the presence of chlorine in concentrations 1000 to 2000% higher than that found in chlorinated drinking water."
Reference: Stanford University
By BM Andersen, B Teige, K Hochlin, N Elsebutangen, L Fagerbakk, B Seljordslia, G Syversen
The increasing global problem with spread of rather robust and resistant microbes, triggers the need of good hygienic routines concerning food, water and the environment.
A newly developed surface disinfection system; Activeion Ionator, converts tap water into activated water, which is intended to be used instead of chemical surface disinfectants. Electric charged water passed through an ion exchange membrane activates nano-bubbles that “breaks down dirt, lifts it from the surface, and enables it to be easily wiped away.” In this study, the Activeion Ionator system was tried with ordinary tap water from the dealer’s office. The water was sprayed through the electric charged system onto plates of agar media. After drying in air the plates were placed in incubation for 4 days at 37°C. This procedure was done repeatedly with different sources of tap water, and after disinfection of the system with 7% alcohol.
A rich growth of one single species identified as most comparable with Acidovorax delafieldii in the Maldi-TOF system, was observed on all agar media sprayed with electric charged tap water. Eleven days later on, the study was repeated, using the same spray flask system and tap water from the hospital department. A rich growth of three types of Gram-negative bacteria, most comparable with Acidovorax delafieldii, Acidovorax temperans and Brevundimonas aurantica, was found in activated tap water taken from our department. After disinfection of the spray system with 70% alcohol and rinsing afterwards with tap water from our department, there was no growth at all on agar media sprayed with activated tap water.
Ionized water produced by the Activeion Ionator system does not kill tough, aquatic bacteria that may contaminate the system. The use of this system may increase the risk of spreading resistant, potential harmful bacteria to the surfaces and into the air. The control of different disinfection equipment for sale is important.
J Microb Biochem Technol 4: 082-085. doi:10.4172/1948- 5948.1000075
Copyright: © 2012 Andersen BM, et al.
BM Andersen - 1*
B Teige - 1
K Hochlin - 1
N Elsebutangen - 1
L Fagerbakk - 1
B Seljordslia -1
G Syversen - 2
1 - Department of Hospital Infections, Oslo University Hospital-Ulleval, Oslo, Norway
2 - Department of Medical Microbiology, Oslo University Hospital-Ulleval, Oslo, Norway
*Corresponding author: BM Andersen, Department of Hospital Infections, Oslo University Hospital-Ulleval, Oslo, Norway, E-mail: email@example.com
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