Show your support of CIRI with the 'CIRI Supporter' logo, available for display on your Web site upon joining CIRI.
Join today and help CIRI advance the cause of cleaner, more productive, and healthier indoor environments through scientific research!
Microbes in the Environment
"[The] ability to inactivate microbes in the environment on a continuous basis is important because bacteria, including MRSA, can survive in a variety of conditions for long periods of time and can spread via dust particles, clothing, furniture, or hospital equipment that have been in contact with infected patients..."
Dr. Charles P. Gerba PhD
Professor of Environmental Microbiology
University of Arizona
By S.M. Zakir Hossain, Cory Ozimok, Clemence Sicard, Sergio D. Aguirre, M. Monsur Ali, Yingfu Li, John D. Brennan
Rapid, sensitive, on-site detection of bacteria without a need for sophisticated equipment or skilled personnel is extremely important in clinical settings and rapid response scenarios, as well as in resource-limited settings. Here, we report a novel approach for selective and ultra-sensitive multiplexed detection of Escherichia coli (non-pathogenic or pathogenic) using a lab-on-paper test strip (bioactive paper) based on intracellular enzyme (Beta-galactosidase (B-GAL) or Beta-glucuronidase (GUS)) activity. The test strip is composed of a paper support (0.5×8 cm), onto which either 5-bromo-4-chloro-3-indolyl-Beta-D-glucuronide sodium salt (XG), chlorophenol red Beta-galactopyranoside (CPRG) or both and FeCl3 were entrapped using sol– gel-derived silica inks in different zones via an ink-jet printing technique. The sample was lysed and assayed via lateral flow through the FeCl3 zone to the substrate area to initiate rapid enzyme hydrolysis of the substrate, causing a change from colorless-to-blue (XG hydrolyzed by GUS, indication of nonpathogenic E. coli) and/or yellow to red-magenta (CPRG hydrolyzed by B-GAL, indication of total coliforms). Using immunomagnetic nanoparticles for selective preconcentration, the limit of detection was ~5 colony-forming units (cfu) per milliliter for E. coli O157:H7 and ~20 cfu/mL for E. coli BL21, within 30 min without cell culturing. Thus, these paper test strips could be suitable for detection of viable total coliforms and pathogens in bathing water samples. Moreover, inclusion of a culturing step allows detection of less than 1 cfu in 100 mL within 8 h, making the paper tests strips relevant for detection of multiple pathogens and total coliform bacteria in beverage and food samples.
Analytical and Bioanalytical Chemistry
Volume 403, Number 6 (2012), 1567-1576, DOI: 10.1007/s00216-012-5975-x
S.M. Zakir Hossain
Sergio D. Aguirre
M. Monsur Ali
John D. Brennan
Department of Chemistry and Chemical Biology
1280 Main St. West
Hamilton, ON L8S 4M1, Canada
Department of Biochemistry and Biomedical Sciences
1200 Main St. West
Hamilton, ON L8N 3Z5, Canada
The Cleaning Industry Research Institute (CIRI) is a 501.c.3 not-for-profit scientific, educational and research organization that applies science to the practice and improvement of cleaning and maintenance.
This abstract/brief is presented under the recognized "fair use" doctrine with respect to article copyright and intellectual property. Readers are encouraged to secure the full article from the originating publication source. Articles also may be obtained through a librarian, an information specialist or inter-library loan. In cases where payment is required under copyright it can be processed through a reference library or the Copyright Clearance Center at www.copyright.com.
CIRI provides no warranty, expressed or implied, and assumes no legal liability for the accuracy, completeness or usefulness of any information disclosed on its site. The views and opinions of authors expressed herein do not necessarily reflect those of CIRI principals, executives, science advisors or affiliates.